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Solid-state NMR for metal-containing zeolites: From active sites to reaction mechanism

Xingling Zhao, Jun Xu, Feng Deng

《化学科学与工程前沿（英文）》 2020年 第14卷 第2期   页码 159-187 doi: 10.1007/s11705-019-1885-1

摘要： Metal-containing zeolite catalysts have found a wide range of applications in heterogeneous catalysis. To understand the nature of metal active sites and the reaction mechanism over such catalysts is of great importance for the establishment of structure-activity relationship. The advanced solid-state NMR (SSNMR) spectroscopy is robust in the study of zeolites and zeolite-catalyzed reactions. In this review, we summarize recent developments and applications of SSNMR for exploring the structure and property of active sites in metal-containing zeolites. Moreover, detailed information on host-guest interactions in the relevant zeolite catalysis obtained by SSNMR is also discussed. Finally, we highlight the mechanistic understanding of catalytic reactions on metal-containing zeolites based on the observation of key surface species and active intermediates.

关键词： metal-containing zeolites     solid-state NMR     active site     host-guest interaction     reaction mechanism    

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基于液-液界面的主-客体分子识别 Review

王贝贝, 陈浩, 刘倓, 史少伟, Thomas P. Russell

《工程（英文）》 2021年 第7卷 第5期   页码 603-614 doi: 10.1016/j.eng.2021.02.004

摘要：

由于动态和可逆的性质，主-客体识别作为一种非共价键相互作用，赋予了液-液界面独特的性质，包括刺激响应性和自调节性等。近年来，越来越多的研究致力于通过在液-液界面上集成功能性单元（如胶体粒子、聚合物）来构筑二维薄膜和微胶囊等超分子界面体系，这为物质封装、递送、双相反应器等领域提供了重要的发展机遇。在本文中，我们总结了近年来基于主-客体相互作用构筑超分子界面体系的研究进展，并对其制备方法、功能和应用进行了概述。

关键词： 液-液界面     自组装     主-客体化学     微胶囊    

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Gene and protein expression of proteinase-activated receptor-1, 2 in a murine model of acute graft host

Quan LI MD , Weiming LI MD , Ping ZOU MD , Jian ZHANG BM ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 309-315 doi: 10.1007/s11684-009-0043-4

摘要： Proteinase-activated receptors (PARs) are a novel subclass of seven transmembrane-spanning, G protein-coupled receptors. PAR-1 and PAR-2 are widely expressed in a variety of cells and are found to be involved in many physiological and pathological processes including inflammation and immune response. However, little is known about the function of PAR-1, 2 in acute graft host disease (GVHD). In the present study, we first detected the expression of PAR-1, 2 protein and mRNA in a murine model of acute GVHD using the methods of immunohistochemistry, Western blot and quantitative real-time polymerase chain reaction (PCR). Syngeneic hematopoietic stem cell transplantation (HSCT) mice served as controls. The relative gene expression level of PAR-1 was significantly increased in the skin, liver, small intestine of allogeneic HSCT mice (in skin: 0.039±0.013 0.008±0.002 of controls, =0.009; in liver: 0.165±0.006 0.017±0.006 of controls, =0.004; in small intestine: 0.215±0.009 0.016±0.002 of controls, =0.003), but not in the stomach, lung and kidney of allogeneic HSCT mice (>0.05). PAR-2 mRNA expression in the liver and small intestine of allogeneic HSCT mice (in liver: 0.010±0.002 0.003±0.001 of controls, =0.008; in small intestine: 0.006±0.001 0.003±0.001 of controls, =0.024) was increased significantly, but PAR-2 mRNA expression in the other organs (>0.05) was not found to be significantly elevated. PAR-1, 2 protein expression was in accordance with the mRNA expression, as shown by Western blot. Using immunohistochemistry the present study demonstrated that there was strong PAR-1, 2 immunoreactivity in the epithelial cell and vascular endothelial cell of target organs of acute GVHD. Our findings of markedly increased expression of PAR-1, 2 in target organs of acute GVHD suggest that PAR-1 and PAR-2 may play an important role in the pathogenesis of acute GVHD.

关键词： graft vs host disease     proteinase-activated receptor     murine model     hematopoietic stem cell transplantation    

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封装分子识别纳米凝胶的功能胶囊用于简捷有效移除水中有机微污染物 Article

刘文英, 巨晓洁, 蒲兴群, 蔡泉威, 刘玉琼, 刘壮, 汪伟, 谢锐, 褚良银

《工程（英文）》 2021年 第7卷 第5期   页码 636-646 doi: 10.1016/j.eng.2021.02.007

摘要：

本文提出了一种利用封装具有分子识别特性纳米凝胶的功能胶囊简捷有效移除水中有机微污染物（OMP）的新方法。该功能胶囊由具有半透膜特性的海藻酸钙（Ca-Alg）凝胶囊膜和具有分子识别特性的聚[N-异丙基丙烯酰胺-共聚-丙烯酸-接枝-单（6-乙二胺基-6-去氧）-β-环糊精]（PNCD）纳米凝胶组成。其中，具有半透膜特性的Ca-Alg凝胶囊膜可以使OMP和水分子自由跨膜传输进入囊的内部，而截留封装的PNCD纳米凝胶。以具有毒性且在水中分布较广的双酚A（BPA）作为模型OMP，基于主-客体识别络合作用，PNCD纳米凝胶上的CD基团能够有效捕捉BPA分子。基于此，将功能胶囊置于含有BPA的水溶液中，即可实现水中OMP的简捷有效移除。另外，由于功能胶囊具有毫米尺度，进一步利用滤网即可便捷地将胶囊从溶液中分离出来。该功能胶囊对BPA的吸附动力学符合拟二级动力学模型，且其等温吸附热力学同时满足Freundlich以及Langmuir等温吸附模型。另外，由于所制备的PNCD纳米凝胶具有温敏性，进一步利用纯水，在高于PNCD纳米凝胶的体积相变温度的条件下，反复清洗胶囊即可实现胶囊的循环再生利用。因此，本研究提出的方法
为简捷有效地移除水中OMP提供了一种新的策略。

关键词： 功能胶囊     分子识别纳米凝胶     有机微污染物     主–客体络合     分离    

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Integrated analysis of gut microbiome and host immune responses in COVID-19

《医学前沿（英文）》 2022年 第16卷 第2期   页码 263-275 doi: 10.1007/s11684-022-0921-6

摘要： Emerging evidence indicates that the gut microbiome contributes to the host immune response to infectious diseases. Here, to explore the role of the gut microbiome in the host immune responses in COVID-19, we conducted shotgun metagenomic sequencing and immune profiling of 14 severe/critical and 24 mild/moderate COVID-19 cases as well as 31 healthy control samples. We found that the diversity of the gut microbiome was reduced in severe/critical COVID-19 cases compared to mild/moderate ones. We identified the abundance of some gut microbes altered post-SARS-CoV-2 infection and related to disease severity, such as Enterococcus faecium, Coprococcus comes, Roseburia intestinalis, Akkermansia muciniphila, Bacteroides cellulosilyticus and Blautia obeum. We further analyzed the correlation between the abundance of gut microbes and host responses, and obtained a correlation map between clinical features of COVID-19 and 16 severity-related gut microbe, including Coprococcus comes that was positively correlated with CD3+/CD4+/CD8+ lymphocyte counts. In addition, an integrative analysis of gut microbiome and the transcriptome of peripheral blood mononuclear cells (PBMCs) showed that genes related to viral transcription and apoptosis were up-regulated in Coprococcus comes low samples. Moreover, a number of metabolic pathways in gut microbes were also found to be differentially enriched in severe/critical or mild/moderate COVID-19 cases, including the superpathways of polyamine biosynthesis II and sulfur oxidation that were suppressed in severe/critical COVID-19. Together, our study highlighted a potential regulatory role of severity related gut microbes in the immune response of host.

关键词： COVID-19     SARS-COV-2     gut microbiome     immune response    

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Graft versus host disease after liver transplantation: A case report

Peng-Ji GAO, Xi-Sheng LENG, Dong WANG, Guang-Ming LI, Lei HUANG, Jie GAO, JI-Ye ZHU,

《医学前沿（英文）》 2010年 第4卷 第4期   页码 469-472 doi: 10.1007/s11684-010-0120-8

摘要： In documenting clinical experience in the diagnosis and treatment of graft host disease (GVHD), we retrospectively analyzed data of one case that has developed GVHD after liver transplantation. This patient exhibited fever, skin rash, and diarrhea on day 9 after liver transplantation. His liver function was normal. Skin biopsy showed scattered keratinocytes accompanied by satellite-like lymphocyte infiltration and basal cell liquefaction degeneration. After carefully analyzing the complications, we took the strategy of decreasing the dose of tacrolimus. Thereafter, the patient’s temperature decreased to normal, his skin rashes subsided, and his diarrhea was relieved. This case suggests that reducing the dosage of immunosuppressive agents can be an effective strategy for GVHD after liver transplantation.

关键词： liver transplantation     graft versus host disease    

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INTERFERENCE BY NON-HOST PLANT ROOTS AND ROOT EXUDATES IN THE INFECTION PROCESSES OF PHYTOPHTHORA NICOTIANAE

《农业科学与工程前沿（英文）》 2021年 第8卷 第3期   页码 447-459 doi: 10.15302/J-FASE-2021399

摘要：

Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases. The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in crop rotations. Interactions between fennel (Foeniculum vulgare) roots/root exudates and Phytophthora nicotianae were investigated because of the known allelopathy between fennel and tobacco (Nicotiana tabacum). The effects of the key compounds in the fennel rhizosphere on the mycelial growth and zoospore behavior of P. nicotianae were assessed. The roots of fennel attracted P. nicotianae zoospores and inhibited their motility and the germination of cystospores, with some cystospores rupturing. 4-ethylacetophenone, vanillin and N-formylpiperidine were consistently identified in the fennel rhizosphere and were found to interfere with the infection of P. nicotianae, especially vanillin. Hyphae treated with these compounds produced more abnormal branches and accumulated reactive oxygen species. These interspecific interactions between non-host roots and pathogens were found to be an important factor in the inhibition by fennel of infection by P. nicotianae.

 

关键词： fennel and tobacco rotation     infection behavior     Phytophthora nicotianae     reactive oxygen species     vanillin    

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Permeability and thermal conductivity of host compressed natural graphite for consolidated activated

Bo TIAN, Liwei WANG, Zhequan JIN, Ruzhu WANG

《能源前沿（英文）》 2011年 第5卷 第2期   页码 159-165 doi: 10.1007/s11708-011-0145-y

摘要： Permeability and thermal conductivity test units were set up to study the heat and mass transfer performance of the host material, i.e. expanded natural graphite (ENG), for consolidated activated carbon (AC) adsorbent. The permeability was tested with nitrogen as the gas source, and the thermal conductivity was studied using steady-state heat source method. The results showed that the values of permeability and thermal conductivity were 10 to 10 m and 1.7 to 3.2 W/(m·K), respectively, while the density compressed expanded natural graphite (CENG) varied from 100 to 500 kg/m . The permeability decreased with the increasing density of CENG, whereas the thermal conductivity increased with the increasing density of CENG. Then the thermal conductivity and permeability of granular AC were researched. It was discovered that the thermal conductivity of samples with different grain size almost kept constant at 0.36 W/(m·K) while the density was approximately 600 kg/m . This means that the thermal conductivity was not related to the grain size of AC. The thermal conductivity of CENG was improved by 5 to 10 times compared with that of granular AC. Such a result showed that CENG was a promising host material for AC to improve the heat transfer performance, while the mass transfer performance should be considered in different conditions for utilization of adsorbent.

关键词： permeability     thermal conductivity     expanded nature graphite     activated carbon    

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Host protection against Omicron BA.2.2 sublineages by prior vaccination in spring 2022 COVID-19 outbreak

《医学前沿（英文）》 2023年 第17卷 第3期   页码 562-575 doi: 10.1007/s11684-022-0977-3

摘要： The Omicron family of SARS-CoV-2 variants are currently driving the COVID-19 pandemic. Here we analyzed the clinical laboratory test results of 9911 Omicron BA.2.2 sublineages-infected symptomatic patients without earlier infection histories during a SARS-CoV-2 outbreak in Shanghai in spring 2022. Compared to an earlier patient cohort infected by SARS-CoV-2 prototype strains in 2020, BA.2.2 infection led to distinct fluctuations of pathophysiological markers in the peripheral blood. In particular, severe/critical cases of COVID-19 post BA.2.2 infection were associated with less pro-inflammatory macrophage activation and stronger interferon alpha response in the bronchoalveolar microenvironment. Importantly, the abnormal biomarkers were significantly subdued in individuals who had been immunized by 2 or 3 doses of SARS-CoV-2 prototype-inactivated vaccines, supporting the estimation of an overall 96.02% of protection rate against severe/critical disease in the 4854 cases in our BA.2.2 patient cohort with traceable vaccination records. Furthermore, even though age was a critical risk factor of the severity of COVID-19 post BA.2.2 infection, vaccination-elicited protection against severe/critical COVID-19 reached 90.15% in patients aged ≥ 60 years old. Together, our study delineates the pathophysiological features of Omicron BA.2.2 sublineages and demonstrates significant protection conferred by prior prototype-based inactivated vaccines.

关键词： SARS-CoV-2     COVID-19     host response     bronchoalveolar lavage fluid (BALF)    

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鱼类肠道菌群研究进展及潜在应用 Review

栾银银, 黎明, 周伟, 药园园, 杨雅麟, 张震, Einar Ringø, Rolf Erik Olsen, Jihong Liu Clarke, 解绶启, 麦康森, 冉超, 周志刚

《工程（英文）》 2023年 第29卷 第10期   页码 137-146 doi: 10.1016/j.eng.2022.12.011

摘要：

肠道菌群在宿主健康和疾病中起着重要作用。我们对鱼类菌群的理解远落后于人类和其他哺乳动物。尽管如此，现有研究也强调了微生物对鱼类的健康、生长性能和各种生理功能中的重要性。目前，模式动物、经济鱼类和野生鱼类中都展开了微生物的研究。鱼类菌群的组成取决于宿主选择、饮食和环境等因素。肠道菌群可以影响鱼类宿主的营养代谢、免疫和抗病能力，而宿主则通过免疫和非免疫因素以鱼菌互作的方式调节肠道菌群的稳态。目前已经开发出的无菌鱼类模型，对于研究鱼类与微生物互作的机理研究有着重要意义。在本篇综述中，我们讨论了鱼类微生物研究的最新进展，描述了包括鱼类微生物多样性，以及宿主-菌群交互作用的研究结果。同时，还讨论了鱼类菌群对鱼类健康及行业可持续发展的潜在作用。

关键词： 肠道菌群     鱼     宿主-菌群交互作用     水产养殖    

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Reactive power compensation of an isolated hybrid power system with load interaction using ANFIS tuned

Nitin SAXENA,Ashwani KUMAR

《能源前沿（英文）》 2014年 第8卷 第2期   页码 261-268 doi: 10.1007/s11708-014-0298-6

摘要： This paper presents an adaptive neuro fuzzy interference system (ANFIS) based approach to tune the parameters of the static synchronous compensator (STATCOM) with frequent disturbances in load model and power input of a wind-diesel based isolated hybrid power system (IHPS). In literature, proportional integral (PI) based controller constants are optimized for voltage stability in hybrid systems due to the interaction of load disturbances and input power disturbances. These conventional controlling techniques use the integral square error (ISE) criterion with an open loop load model. An ANFIS tuned constants of a STATCOM controller for controlling the reactive power requirement to stabilize the voltage variation is proposed in the paper. Moreover, the interaction between the load and the isolated power system is developed in terms of closed loop load interaction with the system. Furthermore, a comparison of transient responses of IHPS is also presented when the system has only the STATCOM and the static compensation requirement of the induction generator is fulfilled by the fixed capacitor, dynamic compensation requirement, meanwhile, is fulfilled by STATCOM. The model is tested for a 1% step increase in reactive power load demand at = 0 s and then a sudden change of 3% from the 1% at = 0.01 s for a 1% step increase in power input at variable wind speed model.

关键词： isolated wind-diesel power system     adaptive neuro fuzzy interference system (ANFIS)     integral square error (ISE) criterion     load interaction    

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Risk factors for chronic graft-versus-host disease after anti-thymocyte globulin-based haploidentical

Meng Lv, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang, Xiaodong Mo

《医学前沿（英文）》 2019年 第13卷 第6期   页码 667-679 doi: 10.1007/s11684-019-0702-z

摘要： Chronic graft-versus-host disease (cGVHD) is a major complication following unmanipulated haploidentical hematopoietic stem cell transplantation (haplo-HSCT). We aimed to identify the risk factors for cGVHD in patients who underwent anti-thymocyte globulin-based haplo-HSCT for acute myeloid leukemia ( =280). The diagnosis of cGVHD was in accordance with the National Institutes of Health consensus criteria. A total of 169 patients suffered from cGVHD. The patients who had 3 loci mismatched had a higher 8-year incidence of cGVHD (total, 66.0% vs. 53.7%, =0.031; moderate to severe, 42.4% vs. 30.1%, =0.036) than the patients who had 1 to 2 loci mismatched. The patients who had maternal donors had a higher 8-year incidence of moderate to severe cGVHD (49.2% vs. 32.9%, =0.024) compared with the patients who had other donors. The patients who had grades III to IV acute GVHD (aGVHD) had higher 8-year incidence of cGVHD (total, 88.0% vs. 50.4%, <0.001; moderate to severe, 68.0% vs. 27.0%, <0.001) compared with the patients without aGVHD. In multivariate analysis, grades III to IV aGVHD was the only independent risk factor for cGVHD. Thus, further interventions should be considered in patients with severe aGVHD to prevent cGVHD.

关键词： acute graft-versus-host disease     chronic graft-versus-host disease     National Institutes of Health consensus criteria     acute myeloid leukemia     anti-thymocyte globulin    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 204-207 doi: 10.1007/s11684-010-0043-4

摘要： Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

关键词： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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An efficient method for the dynamic interaction of open structure-foundation systems

LIU Jingbo, GU Yin, LI Bin, WANG Yan

《结构与土木工程前沿（英文）》 2007年 第1卷 第3期   页码 340-345 doi: 10.1007/s11709-007-0045-8

摘要： The structure-foundation system is transformed from an original open system into an approximately hermetic system through a viscous-spring artificial boundary. In addition, the seismic wave scattering problem is changed to a wave source problem by employing an equivalent seismic wave input method. Subsequently, the modal superposition method is adopted to solve the approximated hermetic system. This procedure is highly efficient in analyzing dynamic structure-foundation interaction problems in the time domain. Two numerical examples are presented to illustrate the characteristics of the proposed method: one is a wave source problem in 2-dimensions (2-D) elastic semi-infinite space, and the other is a dynamic structure-foundation interaction problem under seismic excitations. Compared with the traditional direct step-by-step integration method, the proposed method, with a sufficient number of modes included, can significantly reduce the computational time with almost the same precision. The results also indicate that the proposed method is more advantageous for solving large structure-foundation systems of many degrees of freedom.

关键词： structure-foundation interaction     superposition     interaction problem     freedom     efficient    

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标题 作者 时间 类型 操作

Solid-state NMR for metal-containing zeolites: From active sites to reaction mechanism

Xingling Zhao, Jun Xu, Feng Deng

期刊论文

基于液-液界面的主-客体分子识别

王贝贝, 陈浩, 刘倓, 史少伟, Thomas P. Russell

期刊论文

Gene and protein expression of proteinase-activated receptor-1, 2 in a murine model of acute graft host

Quan LI MD , Weiming LI MD , Ping ZOU MD , Jian ZHANG BM ,

期刊论文

封装分子识别纳米凝胶的功能胶囊用于简捷有效移除水中有机微污染物

刘文英, 巨晓洁, 蒲兴群, 蔡泉威, 刘玉琼, 刘壮, 汪伟, 谢锐, 褚良银

期刊论文

Integrated analysis of gut microbiome and host immune responses in COVID-19

期刊论文

朱奎：细菌–细胞互作介导的宿主导向抗菌策略（2022年8月31日）

2022年10月17日

会议视频

Graft versus host disease after liver transplantation: A case report

Peng-Ji GAO, Xi-Sheng LENG, Dong WANG, Guang-Ming LI, Lei HUANG, Jie GAO, JI-Ye ZHU,

期刊论文

INTERFERENCE BY NON-HOST PLANT ROOTS AND ROOT EXUDATES IN THE INFECTION PROCESSES OF PHYTOPHTHORA NICOTIANAE

期刊论文

Permeability and thermal conductivity of host compressed natural graphite for consolidated activated

Bo TIAN, Liwei WANG, Zhequan JIN, Ruzhu WANG

期刊论文

Host protection against Omicron BA.2.2 sublineages by prior vaccination in spring 2022 COVID-19 outbreak

期刊论文

鱼类肠道菌群研究进展及潜在应用

栾银银, 黎明, 周伟, 药园园, 杨雅麟, 张震, Einar Ringø, Rolf Erik Olsen, Jihong Liu Clarke, 解绶启, 麦康森, 冉超, 周志刚

期刊论文

Reactive power compensation of an isolated hybrid power system with load interaction using ANFIS tuned

Nitin SAXENA,Ashwani KUMAR

期刊论文

Risk factors for chronic graft-versus-host disease after anti-thymocyte globulin-based haploidentical

Meng Lv, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Wei Han, Fengrong Wang, Jingzhi Wang, Kaiyan Liu, Xiaojun Huang, Xiaodong Mo

期刊论文

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

期刊论文

An efficient method for the dynamic interaction of open structure-foundation systems

LIU Jingbo, GU Yin, LI Bin, WANG Yan

期刊论文